Yeast Protein Expression Service
Pichia pastoris expression platform with codon optimization and high-density fermentation – from gene to purified protein.
Mut⁺ (methanol utilization fast), AOX1 high-expression, ideal for most recombinant proteins.
Mutˢ slow methanol utilization, lower methanol consumption, suitable for toxic proteins.
Wild-type Zeocin resistance, high-copy integration, stable yields.
Protease-deficient strain, reduces product degradation, improves integrity.
| Step | Content | Lead Time |
|---|---|---|
| Step 1 | Gene synthesis & codon optimization (for Pichia pastoris) | 2–4 weeks |
| Step 2 | Vector construction & subcloning (pPICZα, pPIC9K etc.) | 1 week |
| Step 3 | Transformation & positive clone screening (high-copy/antibiotic gradient) | 2–3 weeks |
| Step 4 | Small-scale expression (shake flask, SDS-PAGE/WB verification) | 1–2 weeks |
| Step 5 | High-density fermentation & purification (1L–100L optional) | 2–4 weeks |
| Total | 7–10 weeks (including expression, purification & QC) | |
Shake Flask
200 ml – 1 L scale
mg range (1–50 mg/L, protein-dependent)
Bioreactor / Fermenter
5 L – 100 L scale
hundreds mg to grams (typical 0.1–2 g/L)
Industrial Fermentation
500 L and above
gram+ level for pre-clinical/industrial supply
Well suited
- Secreted proteins / soluble intracellular proteins
- Proteins requiring disulfide bonds & glycosylation
- Enzymes, cytokines, growth factors
- Antibody fragments (scFv, Fab)
- Vaccine antigens & industrial enzymes
More challenging (custom strategy needed)
- Very large proteins (>150 kDa)
- Complex human-like glycosylation (glyco-engineered strains available)
- Multi-pass transmembrane proteins (membrane protein optimization)
- Host-toxic proteins (special induction / conditional expression)
Cytokine Interleukin analogue (~18 kDa) secreted in Pichia pastoris; fermenter yield >200 mg/L, single-step affinity purity >95%, active confirmed by ELISA.
Single-chain antibody (scFv) Tumor-targeting scFv: shake-flask screening ~12 mg/L; after optimization 5L fermenter reached 110 mg/L, recovery 80%.
Industrial enzyme Thermostable cellulase (~55 kDa) at 500 L scale; wet cell weight >300 g/L; purified specific activity 420 U/mg – suitable for industrial applications.
Above data represent typical project outcomes; actual yields depend on specific sequences & structure. Full QC from cloning to final protein provided.