| Product SKU | BCACP-001253 |
| Product Description | Recombinant Mouse Tumor necrosis factor (Tnf) protein is expressed from E.coli with Tag-Free. It contains 89-235aa. [Accession | P06804]. |
| Uniprot No. | P06804 |
| Gene Names | Tnf |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 89-235aa |
| Species | Mus musculus (Mouse) |
| Tag Info | Tag-Free |
| Molecular weight | 16.4 kDa |
| Activity | The ED50 as determined by a cytotoxicity assay using HT-29 cells is 17 pg/ml. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Tnf; Tnfa; Tnfsf2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; N-terminal fragment; NTF); Intracellular domain 1; ICD1); Intracellular domain 2; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor; soluble form] |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
