| Product SKU | BCACP-000636 |
| Product Description | Recombinant Mouse Prolyl endopeptidase FAP (Fap) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 26-761aa. [Accession | P97321]. |
| Uniprot No. | P97321 |
| Gene Names | Fap |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Expression System | Mammalian cell |
| Expression Region | 26-761aa |
| Species | Mus musculus (Mouse) |
| Tag Info | C-terminal 10xHis-tagged |
| Molecular weight | 91.5 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Human FLT3LG at 2 μg/mL can bind Human FLT3.The EC50 is 1.04-1.28 μg/mL.;Human FLT3LG captured on Protein A Chip can bind Human FLT3 with an affinity constant of 9.23 nM as detected by MetaSPR Assay (WeSPRTM 200). |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Prolyl endopeptidase FAP; EC 3.4.21.26; Dipeptidyl peptidase FAP; EC 3.4.14.5; Fibroblast activation protein alpha; FAPalpha; Gelatine degradation protease FAP; EC 3.4.21.-; Integral membrane serine protease; Post-proline cleaving enzyme; Serine integral membrane protease; SIMP; Surface-expressed protease; Seprase [Cleaved into: Antiplasmin-cleaving enzyme FAP, soluble form; APCE; EC 3.4.14.5; EC 3.4.21.-; EC 3.4.21.26] |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
