| Product SKU | BCACP-000041 |
| Product Description | Recombinant Human Tumor necrosis factor (TNF) protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 77-233aa. [Accession | P01375]. |
| Uniprot No. | P01375 |
| Gene Names | TNF |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | Yeast |
| Expression Region | 77-233aa |
| Species | Homo sapiens (Human) |
| Tag Info | N-terminal 6xHis-tagged |
| Molecular weight | 19.4 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Human TNF at 5 μg/mL can bind Human TNFR2 protein . The EC50 is 3.470-4.107 ng/mL. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | APC1; APC1 protein; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; Tnf; TNF superfamily member 2; TNF superfamily, member 2; TNF, macrophage derived; TNF, monocyte derived; TNF-a; TNF-alpha; TNFA; TNFA_HUMAN; TNFSF2; Tumor necrosis factor (TNF superfamily member 2); Tumor necrosis factor alpha; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor, Membrane Form; Tumor necrosis factor, soluble form |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
