| Product SKU | BCACP-000612 |
| Product Description | Recombinant Human T-cell-specific surface glycoprotein CD28 (CD28) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 19-152aa. [Accession | P10747]. |
| Uniprot No. | P10747 |
| Gene Names | CD28 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | Mammalian cell |
| Expression Region | 19-152aa |
| Species | Homo sapiens (Human) |
| Tag Info | C-terminal 10xHis-tagged |
| Molecular weight | 16.5 kDa |
| Activity | Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.The specific activity is >2000 pmol/min/µg. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | T-cell-specific surface glycoprotein CD28; TP44; CD antigen CD28 |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
