| Product SKU | BCACP-000527 |
| Product Description | Recombinant Human Protein delta homolog 1 (DLK1) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 24-303aa. [Accession | P80370]. |
| Uniprot No. | P80370 |
| Gene Names | DLK1 |
| Purity | Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC. |
| Expression System | Mammalian cell |
| Expression Region | 24-303aa |
| Species | Homo sapiens (Human) |
| Tag Info | C-terminal 10xHis-tagged |
| Molecular weight | 31.2 kDa |
| Activity | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 7.716-15.52 pg/mL.;Measured by its binding ability in a functional ELISA.Immobilized Rat Tnf at 4 μg/mL can bind Human TNFRSF1A. The EC50 is 6.174-7.463 ng/mL.;Measured by its binding ability in a functional ELISA.Immobilized Rat Tnf at 4 μg/mL can bind Rat Tnfrsf1a. The EC50 is 66.99-76.40 ng/mL.;Human TNFRSF1A(MP023977HU1) captured on Protein A Chip can bind Recombinant Rat Tnf with an affinity constant of 0.139 nM as detected by MetaSPR Assay (WeSPRTM 200). |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Protein delta homolog 1; DLK-1; pG2; DLK1; DLK |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
