| Product SKU | BCACP-000596 |
| Product Description | Recombinant Human Insulin-like growth factor II (IGF2) (E30R, F43L, S63P) protein is expressed from Mammalian cell with N-terminal 6xHis-tagged. It contains 25-180aa(E30R,F43L,S63P). [Accession | P01344]. |
| Uniprot No. | P01344 |
| Gene Names | IGF2 |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Expression System | Mammalian cell |
| Expression Region | 25-180aa(E30R,F43L,S63P) |
| Species | Homo sapiens (Human) |
| Tag Info | N-terminal 6xHis-tagged |
| Molecular weight | 20.1 kDa |
| Activity | Measured by its binding ability in a functional ELISA.Immobilized Human IL3RA at 2 μg/mL can bind Anti-IL3RA recombinant antibody.The EC50 is 1.254-1.412 ng/mL.;IL3RA Recombinant Monoclonal Antibody captured on Protein A Chip can bind Human IL3RA with an affinity constant of 0.202 nM as detected by MetaSPR Assay (WeSPRTM 200). |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Insulin-like growth factor 2; Insulin-like growth factor II; IGF-II; Somatomedin-A; T3M-11-derived growth factor [Cleaved into: Insulin-like growth factor II Ala-25 Del; Preptin] |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
