| Product SKU | BCACP-000586 |
| Product Description | Recombinant Human Glypican-3 (GPC3) protein is expressed from Mammalian cell with N-terminal mFc-tagged. It contains 524-563aa. [Accession | P51654]. |
| Uniprot No. | P51654 |
| Gene Names | GPC3 |
| Purity | Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC. |
| Expression System | Mammalian cell |
| Expression Region | 524-563aa |
| Species | Homo sapiens (Human) |
| Tag Info | N-terminal mFc-tagged |
| Molecular weight | 32.1 kDa |
| Activity | Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >400 pmol/min/µg. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7 [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit] |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
