| Product SKU | BCACP-000025 |
| Product Description | Recombinant Human C-C chemokine receptor type 4 (CCR4) protein is expressed from in vitro E.coli expression system with N-terminal 10xHis-tagged. It contains 1-360aa. [Accession | P51679]. |
| Uniprot No. | P51679 |
| Gene Names | CCR4 |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Expression System | in vitro E.coli expression system |
| Expression Region | 1-360aa |
| Species | Homo sapiens (Human) |
| Tag Info | N-terminal 10xHis-tagged |
| Molecular weight | 44.2 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CCR4 at 5 μg/mL can bind Anti-CCR4 recombinant antibody . The EC50 is 2.803-3.369 ng/mL.;Human CCR4 Full Length Protein captured on CM5 chip can bind Human CCR4 Monoclonal Antibody with an affinity constant of 3.16 nM as detected by SPR Assay(Biacore T200).;Human CCR4 Monoclonal Antibody captured on Protein A Chip can bind Human CCR4 Full Length Protein with an affinity constant of 1.71 nM as detected by MetaSPR Assay (in presence of FOS-12)(WeSPRTM200) . |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4; K5-5; CD194 |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
