| Product SKU | BCACP-001116 |
| Product Description | Recombinant Bovine Fibroblast growth factor 2 (FGF2) protein is expressed from E.coli with Tag free. It contains 10-155aa. [Accession | P03969]. |
| Uniprot No. | P03969 |
| Gene Names | bFGF/FGF-2 |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 10-155aa |
| Species | Bos taurus (Bovine) |
| Tag Info | Tag free |
| Molecular weight | 16.5 kDa |
| Activity | Measured in a cell proliferation assay using B16-F1 cells. Human Fetuin A stimulates adhesion of B16-F1 cells. The ED50 for this effect is 50-200 μg/mL.Optimal concentration depends on cell type as well as the application or research objectives. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
