Recombinant Bovine Coagulation factor IX (F9) (Active)

SKU: BCACP-000374 Category:

Product Specifications

Product SKUBCACP-000374
Product DescriptionRecombinant Bovine Coagulation factor IX (F9) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 26-462aa. [Accession | P00741].
Uniprot No.P00741
Gene NamesF9
PurityGreater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region26-462aa
SpeciesBos taurus (Bovine)
Tag InfoC-terminal 10xHis-tagged
Molecular weight50.7 kDa
ActivityMeasured by its binding ability in a functional ELISA.Immobilized Mouse Lypd3 at 2 μg/mL can bind Anti-LYPD3 recombinant antibody . The EC50 is 1.995-2.345 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCoagulation factor IX; EC 3.4.21.22; Christmas factor [Cleaved into: Coagulation factor IXa light chain; Coagulation factor IXa heavy chain]

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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