Protein Expression Service
With more than 2,000 new protein development capabilities per year, Biocrest Science has extensive experience in the production of recombinant proteins such as transmembrane proteins, enzymes, kinases and viral antigens, relying on advanced protein expression technologies. The proteins delivered by Biocrest Science can be used in drug research, antibody preparation, animal experiments, ELISA, crystal structure studies, in vitro enzyme activity determination, cell experiments, etc., to meet the different upstream and downstream needs of customers. Biocrest Science has been committed to recombinant protein expression and preparation research for many years, and has a variety of protein expression systems: E. coli expression system, yeast cell expression system, insect baculovirus expression system, mammalian cell expression system, and cell-free expression system. The ability to quickly customize protein expression strategies for different customers, while providing customers with a variety of protein production specifications, combined with our unique protein purification platform, can obtain high quality recombinant protein products in a short period of time.
8,000+
Successful cases
200mg/L
High yield
μg-kg
Production scale
98%
Success rate
Advantages of Biocrest Science
- One-stop services from gene synthesis, vector construction, and protein expression to protein purification.
- The ability to develop more than 500 new proteins every year, including viral proteins, cytokines, drug targets and enzymes.
- Independently developed more than 10,000ug to gram grade recombinant proteins.
- Committed to providing customers with high activity, low cost recombinant protein.
- Strict quality control system ensures batch-to-batch consistency and reproducibility
- Four protein expression systems, including prokaryotic, yeast cell expression, insect cells, mammalian cells, perfect protein expression technology platform and quality control system.
Recombinant Protein Expression Systems
Bacterial Protein Expression Service
Insect Baculovirus Expression Service
Yeast Protein Expression Service
Mammalian Protein Expression Service
As fast as 3 weeks delivery;
Simple system optimization;
Clear genetic hereditary background, low cost and high expression;
Most cost-effective eukaryotic protein expression system;
Inexpensive medium and less demanding culture conditions;
Large-scale bioreactors, sufficient for gram-level protein production, suitable for industrial scale-up;
Easy to express heterologous multimeric proteins, high safety;
Use of virus for transfection, easy to operate, transfection system is easy to form;
Used to express full-length transmembrane proteins with high purity;
Expressed using either transient or stable transitions;
Used for in vitro recombinant expression needs of natural conformational proteins;
Expression product can secrete extracellularly, facilitates the purification of the target protein;
Service process
Expression System Selection Guide
Frequently Asked Questions
1. How to choose the right expression system?
Depends on protein complexity, required modifications, and application. Bacterial systems are fast and low-cost but lack glycosylation. Mammalian systems provide native conformation and PTMs but are more expensive and time-consuming.
2. What is codon optimization?
Adjusting gene sequence to match host codon preference without changing the protein. Improves expression efficiency and yield.
3. How do you control purity and endotoxin?
Affinity purification followed by ion exchange or SEC. Endotoxin removed via specialized resins, validated by LAL method. Typical purity >90%, endotoxin <1.0 EU/μg.
4. What tags are available?
His, GST, Flag, MBP. Tag-free also available upon request.
5. Can you handle difficult proteins (membrane, toxic, etc.)?
Yes. Six proprietary platforms (VLPs, Nanodisc, Cell-free, etc.) specifically designed for challenging targets.