| Product SKU | BCACP-000614 |
| Product Description | Recombinant Human Complement C5 (C5) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 19-1676aa. [Accession | P01031]. |
| Uniprot No. | P01031 |
| Gene Names | C5 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | Mammalian cell |
| Expression Region | 19-1676aa |
| Species | Homo sapiens (Human) |
| Tag Info | C-terminal 10xHis-tagged |
| Molecular weight | 188.0 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Bovine HGF at 2 μg/mL can bind Anti-HGF recombinant antibody . The EC50 is 38.88-61.07 ng/mL.;Measured by its binding ability in a functional ELISA.Immobilized Bovine HGF at 2 μg/mL can bind Human MET . The EC50 is 2.471-2.843 ng/mL.;HGF Recombinant Monoclonal Antibody captured on Protein A Chip can bind Bovine HGF with an affinity constant of 1.73 nM as detected by MetaSPR Assay (WeSPRTM 200).;Recombinant Human MET captured on Protein A Chip can bind Recombinant Bovine HGF with an affinity constant of 0.643 nM as detected by MetaSPR Assay (WeSPRTM 200). |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Complement C5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 [Cleaved into: Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5b; Complement C5 alpha' chain] |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
