| Product SKU | BCACP-000442 |
| Product Description | Recombinant Rat Alkaline phosphatase (Alpp) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 19-501aa. [Accession | D4ADM6]. |
| Uniprot No. | D4ADM6 |
| Gene Names | Alpp |
| Purity | Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC. |
| Expression System | Mammalian cell |
| Expression Region | 19-501aa |
| Species | Rattus norvegicus (Rat) |
| Tag Info | C-terminal 10xHis-tagged |
| Molecular weight | 53.9 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis F3 at 2 μg/mL can bind Anti-F3 recombinant antibody . The EC50 is 0.4495-0.5538 ng/mL. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Alkaline phosphatase; EC:3.1.3.1; Alpp |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
